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M9480443.TXT
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1994-08-20
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Document 0443
DOCN M9480443
TI In vivo induction of human immunodeficiency virus type 1-specific
cytotoxic T lymphocytes and delayed-type hypersensitivity by a 23-amino
acid peptide from the highly conserved region in major core protein p24.
DT 9410
AU Nishino Y; Kameoka M; Okada Y; Zhong Q; Kimura T; Azuma I; Ikuta K;
Institute of Immunological Science, Hokkaido University, Sapporo,;
Japan.
SO Vaccine. 1994 May;12(6):485-91. Unique Identifier : AIDSLINE
MED/94310824
AB Cell-mediated immune responses are a major immune defence mechanism
against the spread of human immunodeficiency virus type 1 (HIV-1) which
may lead to acquired immune deficiency syndrome (AIDS). Therefore, the
best candidate for a peptide vaccine preventive from the onset of the
disease might be a chain section containing both B- and T-cell epitopes
in regions of conserved sequences between the different HIV-1 isolates.
We previously identified the highly conserved linear B-cell epitope (23
amino acids in the major core protein p24). Since the epitopes of
cytotoxic T lymphocytes (CTLs) can be defined by short synthetic
peptides, we examined whether this highly conserved region can elicit
viral-specific, cell-mediated immune responses. The results showed
specific induction of CD8+ CTLs in mice by immunization with the Gag
13-mer peptide. Lysis of targets is specific since unpulsed cells with
the same MHC haplotype or cells with a different MHC haplotype pulsed
with the peptide were resistant to lysis. This in vivo response induced
by the Gag 23-mer peptide was almost the same as that induced by the
15-amino acid peptide from the HIV-1 Env gp120 which is an
immunodominant domain in the V3 loop. Lymphocyte proliferation of T-cell
fraction from immune spleen cells was observed after in vitro
stimulation with the Gag 23-mer peptide, whereas there was no apparent
lymphocyte proliferation with the Env 15-mer peptide. In addition,
specific antibodies were raised against Gag p24 in mice immunized with
the Gag 23-mer peptide.(ABSTRACT TRUNCATED AT 250 WORDS)
DE Amino Acid Sequence Animal Antigens, CD8/ANALYSIS Cell Line Guinea
Pigs Hypersensitivity, Delayed/*ETIOLOGY HIV Core Protein
p24/*IMMUNOLOGY HIV-1/*IMMUNOLOGY Immunization Lymphocyte
Transformation Mice Mice, Inbred BALB C Molecular Sequence Data
Peptide Fragments/*IMMUNOLOGY Support, Non-U.S. Gov't T-Lymphocytes,
Cytotoxic/*IMMUNOLOGY T4 Lymphocytes/IMMUNOLOGY JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).